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pmlc  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pmlc
    ( A,B ) Representative immunofluorescence images <t>of</t> <t>paxillin</t> and F-actin (phalloidin) ( A ), and <t>pMLC</t> and F-actin (phalloidin) ( B ) immunostains are shown in inverted black and white (ibw) contrast as well as color composites (DAPI signal also included). Scale bar = 10 µm. ( C ) Compared to WT cells (n=243), lamella size is significantly increased in DLC1 KD cells (n=290, p-adjusted<0.001) and DLC1 KO cells (n=242, p-adjusted<0.001), while DLC1 rescue cells (n=57) show significantly lower lamella sizes than both WT (p-adjusted=0.0098) and KO cells (p-adjusted<0.001). ( D ) DLC1 KD cells (n=290) display a significant increase in the total area of focal adhesions per cell (p-adjusted<0.001) compared to WT cells (n=243), while DLC1 KO cells (n=242) do not show a significant difference (p-adjusted=0.09). Rescue cells show a significant decrease in the total area of focal adhesions (p-adjusted<0.001). ANOVA plus Tukey’s honestly significant difference test.
    Pmlc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+pmlc/pmc13068432-322-16-17
    Average 86 stars, based on 1 article reviews
    pmlc - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "GTPase-activating protein DLC1 spatio-temporally regulates Rho signaling"

    Article Title: GTPase-activating protein DLC1 spatio-temporally regulates Rho signaling

    Journal: eLife

    doi: 10.7554/eLife.90305

    ( A,B ) Representative immunofluorescence images of paxillin and F-actin (phalloidin) ( A ), and pMLC and F-actin (phalloidin) ( B ) immunostains are shown in inverted black and white (ibw) contrast as well as color composites (DAPI signal also included). Scale bar = 10 µm. ( C ) Compared to WT cells (n=243), lamella size is significantly increased in DLC1 KD cells (n=290, p-adjusted<0.001) and DLC1 KO cells (n=242, p-adjusted<0.001), while DLC1 rescue cells (n=57) show significantly lower lamella sizes than both WT (p-adjusted=0.0098) and KO cells (p-adjusted<0.001). ( D ) DLC1 KD cells (n=290) display a significant increase in the total area of focal adhesions per cell (p-adjusted<0.001) compared to WT cells (n=243), while DLC1 KO cells (n=242) do not show a significant difference (p-adjusted=0.09). Rescue cells show a significant decrease in the total area of focal adhesions (p-adjusted<0.001). ANOVA plus Tukey’s honestly significant difference test.
    Figure Legend Snippet: ( A,B ) Representative immunofluorescence images of paxillin and F-actin (phalloidin) ( A ), and pMLC and F-actin (phalloidin) ( B ) immunostains are shown in inverted black and white (ibw) contrast as well as color composites (DAPI signal also included). Scale bar = 10 µm. ( C ) Compared to WT cells (n=243), lamella size is significantly increased in DLC1 KD cells (n=290, p-adjusted<0.001) and DLC1 KO cells (n=242, p-adjusted<0.001), while DLC1 rescue cells (n=57) show significantly lower lamella sizes than both WT (p-adjusted=0.0098) and KO cells (p-adjusted<0.001). ( D ) DLC1 KD cells (n=290) display a significant increase in the total area of focal adhesions per cell (p-adjusted<0.001) compared to WT cells (n=243), while DLC1 KO cells (n=242) do not show a significant difference (p-adjusted=0.09). Rescue cells show a significant decrease in the total area of focal adhesions (p-adjusted<0.001). ANOVA plus Tukey’s honestly significant difference test.

    Techniques Used: Immunofluorescence

    Related Articles

    Immunofluorescence:

    Article Title: Gα13 controls pharyngeal endoderm convergence by regulating E-cadherin expression and RhoA activation.
    Article Snippet: .. Immunofluorescence staining was performed in blocking solution (1% BSA, 2% DMSO, 0.1% Triton X-100 in PBS) containing the following primary antibodies: anti-pMLC (1:40, Cell Signaling Technology #3671S, Danvers, MA), anti-ZO-1 (1:200, ThermoFisher Scientific #33-9100, Waltham, MA), anti-E-cadherin (1:400) (Babb and Marrs, 2004), anti-MHC (1:30, DSHB MF20, Iowa city, IA), and anti-N-cadherin (1:500, Abcam ab21126, Cambridge, United Kingdom). .. Secondary antibodies used were: Alexa FluorTM 647-conjugated donkey anti-rabbit IgG (H+L) (1:300, ThermoFisher Scientific #A-31573, D ev el o pm en t • A cc ep te d m an us cr ip t Waltham, MA), Alexa FluorTM 568-conjugated goat anti-mouse IgG (H+L) (1:300, ThermoFisher Scientific #A-11004, Waltham, MA).

    Article Title: Gα 13 controls pharyngeal endoderm convergence by regulating E-cadherin expression and RhoA activation
    Article Snippet: .. Immunofluorescence staining was performed in blocking solution (1% BSA, 2% DMSO, 0.1% Triton X-100 in PBS) containing the following primary antibodies: anti-pMLC (1:40, Cell Signaling Technology, 3671S), anti-ZO-1 (1:200, ThermoFisher Scientific, 33-9100), anti-E-cadherin (1:400) , anti-MHC (1:30, DSHB MF20), and anti-N-cadherin (1:500, Abcam, ab21126). .. Secondary antibodies used were: Alexa Fluor 647-conjugated donkey anti-rabbit IgG (H+L) (1:300, ThermoFisher Scientific, A-31573), Alexa Fluor 568-conjugated goat anti-mouse IgG (H+L) (1:300, ThermoFisher Scientific, A-11004).

    Staining:

    Article Title: Gα13 controls pharyngeal endoderm convergence by regulating E-cadherin expression and RhoA activation.
    Article Snippet: .. Immunofluorescence staining was performed in blocking solution (1% BSA, 2% DMSO, 0.1% Triton X-100 in PBS) containing the following primary antibodies: anti-pMLC (1:40, Cell Signaling Technology #3671S, Danvers, MA), anti-ZO-1 (1:200, ThermoFisher Scientific #33-9100, Waltham, MA), anti-E-cadherin (1:400) (Babb and Marrs, 2004), anti-MHC (1:30, DSHB MF20, Iowa city, IA), and anti-N-cadherin (1:500, Abcam ab21126, Cambridge, United Kingdom). .. Secondary antibodies used were: Alexa FluorTM 647-conjugated donkey anti-rabbit IgG (H+L) (1:300, ThermoFisher Scientific #A-31573, D ev el o pm en t • A cc ep te d m an us cr ip t Waltham, MA), Alexa FluorTM 568-conjugated goat anti-mouse IgG (H+L) (1:300, ThermoFisher Scientific #A-11004, Waltham, MA).

    Article Title: AAMP and MTSS1 Are Novel Negative Regulators of Endothelial Barrier Function Identified in a Proteomics Screen
    Article Snippet: As secondary antibodies, HRP-conjugated goat anti-rabbit and anti-mouse antibodies (Dako, Glostrup, Denmark) were used. .. For immunofluorescent staining, the following primary antibodies were used: anti-VE-cadherin (#2500, Cell Signaling Technology and #MAB9381, R&D systems, Minneapolis, MN, USA), anti-pMLC (#3671, Cell Signaling Technology), anti-myc (#2276 and #2278, Cell Signaling Technology), anti-cortactin (#05-180-I, Merck), and anti-p34-Arc (#07-227-I, Merck). .. Alexa-555 donkey anti-rabbit, Alexa-647 donkey anti-mouse, Alexa-488 donkey anti-mouse, Alexa-555 donkey anti-mouse, and Alexa-647 donkey anti-rabbit (Invitrogen) were used as secondary antibodies.

    Article Title: AAMP and MTSS1 Are Novel Negative Regulators of Endothelial Barrier Function Identified in a Proteomics Screen.
    Article Snippet: As secondary antibodies, HRP-conjugated goat anti-rabbit and anti-mouse antibodies (Dako, Glostrup, Denmark) were used. .. For immunofluorescent staining, the following primary antibodies were used: antiVE-cadherin (#2500, Cell Signaling Technology and #MAB9381, R&D systems, Minneapolis, MN, USA), anti-pMLC (#3671, Cell Signaling Technology), anti-myc (#2276 and #2278, Cell Signaling Technology), anti-cortactin (#05-180-I, Merck), and anti-p34-Arc (#07-227-I, Merck). .. Alexa-555 donkey anti-rabbit, Alexa-647 donkey anti-mouse, Alexa-488 donkey anti-mouse, Alexa-555 donkey anti-mouse, and Alexa-647 donkey anti-rabbit (Invitrogen) were used as secondary antibodies.

    Article Title: Gα 13 controls pharyngeal endoderm convergence by regulating E-cadherin expression and RhoA activation
    Article Snippet: .. Immunofluorescence staining was performed in blocking solution (1% BSA, 2% DMSO, 0.1% Triton X-100 in PBS) containing the following primary antibodies: anti-pMLC (1:40, Cell Signaling Technology, 3671S), anti-ZO-1 (1:200, ThermoFisher Scientific, 33-9100), anti-E-cadherin (1:400) , anti-MHC (1:30, DSHB MF20), and anti-N-cadherin (1:500, Abcam, ab21126). .. Secondary antibodies used were: Alexa Fluor 647-conjugated donkey anti-rabbit IgG (H+L) (1:300, ThermoFisher Scientific, A-31573), Alexa Fluor 568-conjugated goat anti-mouse IgG (H+L) (1:300, ThermoFisher Scientific, A-11004).

    Blocking Assay:

    Article Title: Gα13 controls pharyngeal endoderm convergence by regulating E-cadherin expression and RhoA activation.
    Article Snippet: .. Immunofluorescence staining was performed in blocking solution (1% BSA, 2% DMSO, 0.1% Triton X-100 in PBS) containing the following primary antibodies: anti-pMLC (1:40, Cell Signaling Technology #3671S, Danvers, MA), anti-ZO-1 (1:200, ThermoFisher Scientific #33-9100, Waltham, MA), anti-E-cadherin (1:400) (Babb and Marrs, 2004), anti-MHC (1:30, DSHB MF20, Iowa city, IA), and anti-N-cadherin (1:500, Abcam ab21126, Cambridge, United Kingdom). .. Secondary antibodies used were: Alexa FluorTM 647-conjugated donkey anti-rabbit IgG (H+L) (1:300, ThermoFisher Scientific #A-31573, D ev el o pm en t • A cc ep te d m an us cr ip t Waltham, MA), Alexa FluorTM 568-conjugated goat anti-mouse IgG (H+L) (1:300, ThermoFisher Scientific #A-11004, Waltham, MA).

    Article Title: Gα 13 controls pharyngeal endoderm convergence by regulating E-cadherin expression and RhoA activation
    Article Snippet: .. Immunofluorescence staining was performed in blocking solution (1% BSA, 2% DMSO, 0.1% Triton X-100 in PBS) containing the following primary antibodies: anti-pMLC (1:40, Cell Signaling Technology, 3671S), anti-ZO-1 (1:200, ThermoFisher Scientific, 33-9100), anti-E-cadherin (1:400) , anti-MHC (1:30, DSHB MF20), and anti-N-cadherin (1:500, Abcam, ab21126). .. Secondary antibodies used were: Alexa Fluor 647-conjugated donkey anti-rabbit IgG (H+L) (1:300, ThermoFisher Scientific, A-31573), Alexa Fluor 568-conjugated goat anti-mouse IgG (H+L) (1:300, ThermoFisher Scientific, A-11004).

    Inhibition:

    Article Title: Harnessing semen-derived exosomes for noninvasive fundus drug delivery: A paradigm for exosome-based ocular fundus therapeutics
    Article Snippet: .. Key signaling nodes [e.g., EGFR/phosphorylated EGFR (pEGFR), Src/pSrc, MLCK, and MLC/pMLC] were analyzed to confirm pathway-specific inhibition on barrier integrity; Anti-EGFR (1:1000; Abcam, USA, no. Ab32077), anti-pEGFR (1:1000; Abcam, USA, no. Ab134005 ), anti-Src (1:1000; Abcam, USA, no. Ab109381 ), anti-pSrc (1:1000; Abcam, USA, no. Ab4816), anti-MLCK (1:1000; Abcam, USA, no. Ab314185 ), anti-MLC (1:1000; Abcam, USA, no. Ab92721), anti-pMLC (1:1000; Cell Signaling Technology, USA, no. 3672S), anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1000; Abcam, USA, no. Ab8245). .. Key signaling nodes [e.g., EGFR/phosphorylated EGFR (pEGFR), Src/pSrc, MLCK, and MLC/pMLC] were analyzed to confirm pathway-specific inhibition on barrier integrity; Anti-EGFR (1:1000; Abcam, USA, no. Ab32077), anti-pEGFR (1:1000; Abcam, USA, no. Ab134005 ), anti-Src (1:1000; Abcam, USA, no. Ab109381 ), anti-pSrc (1:1000; Abcam, USA, no. Ab4816), anti-MLCK (1:1000; Abcam, USA, no. Ab314185 ), anti-MLC (1:1000; Abcam, USA, no. Ab92721), anti-pMLC (1:1000; Cell Signaling Technology, USA, no. 3672S), anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1000; Abcam, USA, no. Ab8245).

    Western Blot:

    Article Title: AAMP and MTSS1 Are Novel Negative Regulators of Endothelial Barrier Function Identified in a Proteomics Screen.
    Article Snippet: .. For Western blot analysis, the following primary antibodies were used: anti-AAMP (#PA5-31454, Invitrogen, Waltham, MA, USA), anti-MTSS1 (#93065, Cell Signaling Technology, Danvers, MA, USA), anti-RhoB (#14326-1-AP, Proteintech, Manchester, UK), anitCLASP1 (#ab108620, Abcam, Cambridge, UK), anti-SQSTM1/p62 (#5114, Cell Signaling Technology), anti-GAPDH (#2118, Cell Signaling Technology), anti-VE-cadherin (#2500, Cell Signaling Technology), anti-Rac1 (#610650, BD Transduction Laboratories, Franklin Lakes, NJ, USA), anti-RhoA (#2117, Cell Signaling Technology), anti-Cdc42 (#2466, Cell Signaling Technology), anti-pMLC (#3671, Cell Signaling Technology), anti-myc (#2276, Cell Signaling Technology), anti-cortactin (#05-180-I, Merck, Darmstadt, Germany), anti-p34-Arc (#07-227-I, Merck), and anti-Vinculin (#V9131, Sigma-Aldrich, St. Louis, MO, USA). .. As secondary antibodies, HRP-conjugated goat anti-rabbit and anti-mouse antibodies (Dako, Glostrup, Denmark) were used.

    Article Title: AAMP and MTSS1 Are Novel Negative Regulators of Endothelial Barrier Function Identified in a Proteomics Screen
    Article Snippet: .. For Western blot analysis, the following primary antibodies were used: anti-AAMP (#PA5-31454, Invitrogen, Waltham, MA, USA), anti-MTSS1 (#93065, Cell Signaling Technology, Danvers, MA, USA), anti-RhoB (#14326-1-AP, Proteintech, Manchester, UK), anit-CLASP1 (#ab108620, Abcam, Cambridge, UK), anti-SQSTM1/p62 (#5114, Cell Signaling Technology), anti-GAPDH (#2118, Cell Signaling Technology), anti-VE-cadherin (#2500, Cell Signaling Technology), anti-Rac1 (#610650, BD Transduction Laboratories, Franklin Lakes, NJ, USA), anti-RhoA (#2117, Cell Signaling Technology), anti-Cdc42 (#2466, Cell Signaling Technology), anti-pMLC (#3671, Cell Signaling Technology), anti-myc (#2276, Cell Signaling Technology), anti-cortactin (#05-180-I, Merck, Darmstadt, Germany), anti-p34-Arc (#07-227-I, Merck), and anti-Vinculin (#V9131, Sigma-Aldrich, St. Louis, MO, USA). .. As secondary antibodies, HRP-conjugated goat anti-rabbit and anti-mouse antibodies (Dako, Glostrup, Denmark) were used.



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    ( A,B ) Representative immunofluorescence images <t>of</t> <t>paxillin</t> and F-actin (phalloidin) ( A ), and <t>pMLC</t> and F-actin (phalloidin) ( B ) immunostains are shown in inverted black and white (ibw) contrast as well as color composites (DAPI signal also included). Scale bar = 10 µm. ( C ) Compared to WT cells (n=243), lamella size is significantly increased in DLC1 KD cells (n=290, p-adjusted<0.001) and DLC1 KO cells (n=242, p-adjusted<0.001), while DLC1 rescue cells (n=57) show significantly lower lamella sizes than both WT (p-adjusted=0.0098) and KO cells (p-adjusted<0.001). ( D ) DLC1 KD cells (n=290) display a significant increase in the total area of focal adhesions per cell (p-adjusted<0.001) compared to WT cells (n=243), while DLC1 KO cells (n=242) do not show a significant difference (p-adjusted=0.09). Rescue cells show a significant decrease in the total area of focal adhesions (p-adjusted<0.001). ANOVA plus Tukey’s honestly significant difference test.
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    Image Search Results


    ( A,B ) Representative immunofluorescence images of paxillin and F-actin (phalloidin) ( A ), and pMLC and F-actin (phalloidin) ( B ) immunostains are shown in inverted black and white (ibw) contrast as well as color composites (DAPI signal also included). Scale bar = 10 µm. ( C ) Compared to WT cells (n=243), lamella size is significantly increased in DLC1 KD cells (n=290, p-adjusted<0.001) and DLC1 KO cells (n=242, p-adjusted<0.001), while DLC1 rescue cells (n=57) show significantly lower lamella sizes than both WT (p-adjusted=0.0098) and KO cells (p-adjusted<0.001). ( D ) DLC1 KD cells (n=290) display a significant increase in the total area of focal adhesions per cell (p-adjusted<0.001) compared to WT cells (n=243), while DLC1 KO cells (n=242) do not show a significant difference (p-adjusted=0.09). Rescue cells show a significant decrease in the total area of focal adhesions (p-adjusted<0.001). ANOVA plus Tukey’s honestly significant difference test.

    Journal: eLife

    Article Title: GTPase-activating protein DLC1 spatio-temporally regulates Rho signaling

    doi: 10.7554/eLife.90305

    Figure Lengend Snippet: ( A,B ) Representative immunofluorescence images of paxillin and F-actin (phalloidin) ( A ), and pMLC and F-actin (phalloidin) ( B ) immunostains are shown in inverted black and white (ibw) contrast as well as color composites (DAPI signal also included). Scale bar = 10 µm. ( C ) Compared to WT cells (n=243), lamella size is significantly increased in DLC1 KD cells (n=290, p-adjusted<0.001) and DLC1 KO cells (n=242, p-adjusted<0.001), while DLC1 rescue cells (n=57) show significantly lower lamella sizes than both WT (p-adjusted=0.0098) and KO cells (p-adjusted<0.001). ( D ) DLC1 KD cells (n=290) display a significant increase in the total area of focal adhesions per cell (p-adjusted<0.001) compared to WT cells (n=243), while DLC1 KO cells (n=242) do not show a significant difference (p-adjusted=0.09). Rescue cells show a significant decrease in the total area of focal adhesions (p-adjusted<0.001). ANOVA plus Tukey’s honestly significant difference test.

    Article Snippet: Cells were incubated overnight with the primary antibody 1:250 dilution for paxillin (Abcam ab32084), 1:50 for pMLC (Cell Signaling Antibody #3671).

    Techniques: Immunofluorescence